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recombinant human (rh) tgfβ  (PeproTech)


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    Structured Review

    PeproTech recombinant human (rh) tgfβ

    Recombinant Human (Rh) Tgfβ, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+(rh)+tgf%CE%B2/tgf+%CE%B21/pmc11134877-49-0-5
    Average 90 stars, based on 1 article reviews
    recombinant human (rh) tgfβ - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier"

    Article Title: Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier

    Journal: iScience

    doi: 10.1016/j.isci.2024.109909


    Figure Legend Snippet:

    Techniques Used: Control, Recombinant, Modification, Cell Recovery, Lysis, Reverse Transcription, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Software, Microscopy

    Related Articles

    other:

    Article Title: Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier
    Article Snippet: recombinant human (rh) TGFβ , Peprotech , Cat# 100-21A.

    Article Title: IFN-? Receptor Deficient Donor T cells Mediate Protection from Graft-versus-Host Disease and Preserve Graft-versus-Tumor Responses After Allogeneic Bone Marrow Transplantation
    Article Snippet: In vitro T cell activation and polarization of IL-17-producing cells Unfractionated spleen cells (2 × 10 5 /well) or purified CD4 + cells (5 × 10 4 /well) from WT (IFN-γ +/+ and IFN-γR +/+ ), IFN-γ -/- and IFN-γR -/- mice were cultured in 96 well plates for 3-5 days with 5 μg/ml each of anti-CD3 (145-2D11), anti-CD28 (37.51.1, Invitrogen, Carlsbad, CA) and with 20ng/ml recombinant mouse (rm) IL-6 (Peprotech) and 5ng/ml recombinant human (rh) TGFβ (Peprotech, Rocky Hill, NJ) and then analyzed for intracellular cytokine expression.

    Article Title: IFN-? Receptor Deficient Donor T cells Mediate Protection from Graft-versus-Host Disease and Preserve Graft-versus-Tumor Responses After Allogeneic Bone Marrow Transplantation
    Article Snippet: Unfractionated spleen cells (2 × 10 5 /well) or purified CD4 + cells (5 × 10 4 /well) from WT (IFN-γ +/+ and IFN-γR +/+ ), IFN-γ -/- and IFN-γR -/- mice were cultured in 96 well plates for 3-5 days with 5 μg/ml each of anti-CD3 (145-2D11), anti-CD28 (37.51.1, Invitrogen, Carlsbad, CA) and with 20ng/ml recombinant mouse (rm) IL-6 (Peprotech) and 5ng/ml recombinant human (rh) TGFβ (Peprotech, Rocky Hill, NJ) and then analyzed for intracellular cytokine expression.

    Purification:

    Article Title: Antigen receptor-mediated depletion of FOXP3 in induced regulatory T-lymphocytes via PTPN2 and FOXO1
    Article Snippet: .. CD4 + T-cells were purified by magnetic cell sorting from spleens and lymph nodes and were primed with anti (α)-CD3 (5 μg ml −1 ) for 72 h as described , in Click's RPMI medium (Biochrom) in the presence of αCD28 (clone 37.51; 0.5 μg ml −1 ), recombinant (r) human (h) IL-2 (Proleukin, 100 U ml −1 ) and rh TGFβ (Peprotech; 2 ng ml −1 ), αIL-4 (10% of culture supernatant (SN) of 11B11 cells) and αIFNγ (5 μg ml −1 , purified from SN of XMG1.2 cells). .. After priming, cells were removed from the stimulus, washed and re-cultured for 24 or 48 h in 48 well (3 × 10 5 in 0.5 ml medium) or 12 well (3 × 10 6 in 2 ml medium) culture plates (Greiner) with or without pre-coated αCD3 (5 μg ml −1 ) in the presence of IL-2 and/or TGFβ and IL-6 (Peprotech; 10 ng ml −1 ).

    FACS:

    Article Title: Antigen receptor-mediated depletion of FOXP3 in induced regulatory T-lymphocytes via PTPN2 and FOXO1
    Article Snippet: .. CD4 + T-cells were purified by magnetic cell sorting from spleens and lymph nodes and were primed with anti (α)-CD3 (5 μg ml −1 ) for 72 h as described , in Click's RPMI medium (Biochrom) in the presence of αCD28 (clone 37.51; 0.5 μg ml −1 ), recombinant (r) human (h) IL-2 (Proleukin, 100 U ml −1 ) and rh TGFβ (Peprotech; 2 ng ml −1 ), αIL-4 (10% of culture supernatant (SN) of 11B11 cells) and αIFNγ (5 μg ml −1 , purified from SN of XMG1.2 cells). .. After priming, cells were removed from the stimulus, washed and re-cultured for 24 or 48 h in 48 well (3 × 10 5 in 0.5 ml medium) or 12 well (3 × 10 6 in 2 ml medium) culture plates (Greiner) with or without pre-coated αCD3 (5 μg ml −1 ) in the presence of IL-2 and/or TGFβ and IL-6 (Peprotech; 10 ng ml −1 ).

    Recombinant:

    Article Title: Antigen receptor-mediated depletion of FOXP3 in induced regulatory T-lymphocytes via PTPN2 and FOXO1
    Article Snippet: .. CD4 + T-cells were purified by magnetic cell sorting from spleens and lymph nodes and were primed with anti (α)-CD3 (5 μg ml −1 ) for 72 h as described , in Click's RPMI medium (Biochrom) in the presence of αCD28 (clone 37.51; 0.5 μg ml −1 ), recombinant (r) human (h) IL-2 (Proleukin, 100 U ml −1 ) and rh TGFβ (Peprotech; 2 ng ml −1 ), αIL-4 (10% of culture supernatant (SN) of 11B11 cells) and αIFNγ (5 μg ml −1 , purified from SN of XMG1.2 cells). .. After priming, cells were removed from the stimulus, washed and re-cultured for 24 or 48 h in 48 well (3 × 10 5 in 0.5 ml medium) or 12 well (3 × 10 6 in 2 ml medium) culture plates (Greiner) with or without pre-coated αCD3 (5 μg ml −1 ) in the presence of IL-2 and/or TGFβ and IL-6 (Peprotech; 10 ng ml −1 ).



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    Image Search Results


    Journal: iScience

    Article Title: Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier

    doi: 10.1016/j.isci.2024.109909

    Figure Lengend Snippet:

    Article Snippet: recombinant human (rh) TGFβ , Peprotech , Cat# 100-21A.

    Techniques: Control, Recombinant, Modification, Cell Recovery, Lysis, Reverse Transcription, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Software, Microscopy

    Inflammatory cytokines are upstream regulators of gene expression changes between neonatal and fetal stages and induce increased expression of brush border, absorption, and mucosal defense markers in vitro (A) Ingenuity pathway analysis showed inflammatory cytokines IFNγ, TNFα, IL1β, and TGFβ1 as upstream regulators of intestinal changes between late and early gestational age tissue and between neonatal versus fetal tissue. Activation Z score is presented by number and color. Orange color represents activation status of upstream regulator while blue signifies inhibition. (B–K) RT-qPCR analyses of maturation markers in proximal (red) and distal (blue) small intestine HFOs of (B and C) IAP , (D and E) LCT , (F and G) SIS , (H and I) FABP6 and (J and K) PIGR . n = 12 independent donors, data are plotted as paired values of individual cultures. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 as determined by non-parametric one-way ANOVA. See also <xref ref-type=Figures S2–S4 . " width="100%" height="100%">

    Journal: iScience

    Article Title: Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier

    doi: 10.1016/j.isci.2024.109909

    Figure Lengend Snippet: Inflammatory cytokines are upstream regulators of gene expression changes between neonatal and fetal stages and induce increased expression of brush border, absorption, and mucosal defense markers in vitro (A) Ingenuity pathway analysis showed inflammatory cytokines IFNγ, TNFα, IL1β, and TGFβ1 as upstream regulators of intestinal changes between late and early gestational age tissue and between neonatal versus fetal tissue. Activation Z score is presented by number and color. Orange color represents activation status of upstream regulator while blue signifies inhibition. (B–K) RT-qPCR analyses of maturation markers in proximal (red) and distal (blue) small intestine HFOs of (B and C) IAP , (D and E) LCT , (F and G) SIS , (H and I) FABP6 and (J and K) PIGR . n = 12 independent donors, data are plotted as paired values of individual cultures. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 as determined by non-parametric one-way ANOVA. See also Figures S2–S4 .

    Article Snippet: HFOs donors were stimulated for 72 h with the following cytokines: recombinant human (rh) IFNγ 0,5 ng/ml (R&D systems, cat#285-IF), rhTNFα 2ng/ml (Peprotech, cat#:300-01A), rhIL1β 5 ng/ml (Thermo Fisher Scientific, cat#PHC0814), rhTGFβ 5 ng/ml (Peprotech, cat#:100-21A).

    Techniques: Gene Expression, Expressing, In Vitro, Activation Assay, Inhibition, Quantitative RT-PCR

    IFNγ and cytokine mix stimulation increases IAP activity and FABP6 protein expression in HFOs (A and B) Alkaline phosphatase activity level (μg pNPP/min) in (A) proximal and (B) distal HFOs upon cytokines stimulation ( n = 9 independent donors). (C) Alkaline phosphatase (IAP) staining of embedded HFOs upon IFNγ, and cytokine mix stimulation compared to control. (D) FABP6 protein expression measured by western blot in distal HFOs. (E) FABP6 western blot quantification on n = 5 independent donors. ∗ p < 0.05, ∗∗ p < 0.01 as determined by non-parametric one-way ANOVA.

    Journal: iScience

    Article Title: Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier

    doi: 10.1016/j.isci.2024.109909

    Figure Lengend Snippet: IFNγ and cytokine mix stimulation increases IAP activity and FABP6 protein expression in HFOs (A and B) Alkaline phosphatase activity level (μg pNPP/min) in (A) proximal and (B) distal HFOs upon cytokines stimulation ( n = 9 independent donors). (C) Alkaline phosphatase (IAP) staining of embedded HFOs upon IFNγ, and cytokine mix stimulation compared to control. (D) FABP6 protein expression measured by western blot in distal HFOs. (E) FABP6 western blot quantification on n = 5 independent donors. ∗ p < 0.05, ∗∗ p < 0.01 as determined by non-parametric one-way ANOVA.

    Article Snippet: HFOs donors were stimulated for 72 h with the following cytokines: recombinant human (rh) IFNγ 0,5 ng/ml (R&D systems, cat#285-IF), rhTNFα 2ng/ml (Peprotech, cat#:300-01A), rhIL1β 5 ng/ml (Thermo Fisher Scientific, cat#PHC0814), rhTGFβ 5 ng/ml (Peprotech, cat#:100-21A).

    Techniques: Activity Assay, Expressing, Staining, Control, Western Blot